Figure 8
From: TGF-β-induced hepatocyte lincRNA-p21 contributes to liver fibrosis in mice
LincRNA-p21 promotes hepatocyte apoptosis. (A) Ectopic expression of lincRNA-p21 promotes hepatocyte apoptosis. AML12 cells were transfected with pCI-lincRNA-p21 for 48 h. Cell apoptosis was determined by FACS analysis. (B) Effects of lincRNA-p21 on Bax, Bad and Bcl-XL expression in AML12 cells. (C) lincRNA-p21 promotes caspase-3 cleavage. Caspase-3 and cleaved caspase-3 were detected by western blot. (D) Knockdown of lincRNA-p21 inhibits doxorubicin-induced hepatocyte apoptosis. AML12 cells were transfected with lincRNA-p21 siRNA for 48 h in the presence of 0.8 μg/ml doxorubicin. (E) lincRNA-p21 siRNA inhibits doxorubicin-induced upregulation of lincRNA-p21. (F) Effects of lincRNA-p21 siRNA on doxorubicin-induced deregulation of Bax, Bad and Bcl-XL. (G) Knockdown of lincRNA-p21 inhibits caspase-3 activation. (H) Knockdown of lincRNA-p21 inhibits hepatocyte apoptosis in the CCl4-induced fibrotic livers. Apoptotic hepatocytes were evaluated by TUNEL staining (left) and its quantification (right). Scale bar, 50 μm. (I) Knockdown of lincRNA-p21 decreased serum ALT in the CCl4-treated mice. qRT-PCR results are shown as fold change compared with control group. Data are the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01. The original immunoblot images are shown in Supplementary Figure S11.
