Table 2 Comparison of kinetic parameters of DAAO to D-Pro and L-Pro under different pH values.
From: PH-Dependent Enantioselectivity of D-amino Acid Oxidase in Aqueous Solution
pH | D-Pro | L-Pro | k cat(D-Pro) /k cat(L-Pro) | ||
|---|---|---|---|---|---|
k cat(s−1) | K m(mM) | k cat(s−1) | K m(mM) | ||
7.5 | 50.20 ± 1.20 | 0.38 ± 0.02 | 0.70 ± 0.06 | 252.00 ± 10.00 | 71.42 |
8.0 | 107.30 ± 9.10 | 0.33 ± 0.01 | 6.71 ± 0.51 | 20.00 ± 1.73 | 15.99 |
8.5 | 43.30 ± 3.40 | 0.85 ± 0.06 | 0.40 ± 0.03 | 1800.00 ± 90.50 | 108.25 |
