Figure 1
Wild-type (WT) mice developed cardiac remodeling and lost GTP cyclohydrolase 1 (GCH1) proteins following myocardial infarction (MI). (A) Time-dependent changes in mouse hearts after MI or sham surgery (n = 6–8 mice/group). Top: representative images of mouse hearts at baseline (0), 1, 2, 4, 8, and 12 weeks after operation. The vertical scale bar indicates 4 mm, and the horizontal scale bar indicates 2 mm. Bottom: the area of the hearts after MI or sham surgery; (B) The anterior wall thickness of the left ventricule after MI or sham surgery (n = 8–10 mice/group); (C) The thickness of interventricular septum following MI or sham surgery (n = 8–10 mice/group); (D) Representative PCR bands showing the expression of GCH1 mRNA and the housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), at 0, 1, 2, 4, 8, and 12 weeks after MI; (E) GCH1 mRNA normalized to GAPDH (n = 6 mice/group); (F) Representative Western blot bands showing the expression of cardiac mouse GCH1 proteins and GAPDH proteins in WT mice following MI; (G) Expression of mouse GCH1 proteins normalized to GAPDH proteins (n = 6 mice/group). *P < 0.05 versus sham WT groups at corresponding time points (A,B and C) or 0 weeks (G).
