Figure 5 | Scientific Reports

Figure 5

From: Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells

Figure 5

Passaging of human iPS cells in the core-shell microfiber culture system. (A) Expansion rates of human iPS cells cultured in the microfibers accompanied with collagen at low initial cell density (1.0 × 107 cells/mL) before and after passage (N ≥ 3). The cells were passaged on day 4, 6, or 8. *P < 0.05. (B) Expansion rates of the cells cultured in the microfibers by serial passaging (N ≥ 3). Cells were passaged every 4 days and cultured until 8 passages (32 days).

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