Figure 3
Presence of anti p185neu Abs and in vivo cytotoxicity in HER-2/neu transgenic mice after BI. Sbp of sera to p185 protein (A, top) or in vivo cytotoxicity (A, bottom) from three animals of SI and BI groups were analysed at all time points reported. The ability of sera to bind p185neu (Sbp) was evaluated by flow cytometry as reported in M&M. For cytotoxicity, immunized mice of different age were injected with CFSE stained splenocytes from naıve transgenic mice pulsed with either HER-2 immunodominant or irrelevant peptides and were analyzed by flow cytometry as detailed in M&M.; anti-p185neu IgM or IgGs isotytpes were analysed in three mice of SI and BI groups at 39 weeks of age (B); p185 protein in 104 weeks old-mice of ECD1.5 group with or without tumour are shown (C).
