Figure 5
From: Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
AcHE1b and Zinc (Zn+2) transport. AcHE1b OV immature oocytes were treated with intra (TPEN) or extra (DPTA) cellular Zn+2 chelator and the ratio of intra and extra cellular Zn+2 ion concentration, and AcHE1b, Beclin1 and P53 transcription (A) was measured. ULK1 and LC3 antibodies were used to confirm to extent of autophagy in DMSO (B), TPEN (C) and DPTA (D) treated AcHE1b OV oocytes. Western blotting analysis also substantiated the autophagic/apoptotic alterations (E). Our data suggests that AcHE1b regulates the Zn+2 ion balance, which further decides the cell fate. General mechanism of AcHE-atresia regulation is drawn based on our analysis (F).
