Figure 3

Effect of β-Lap on pancreatic NAD⁺ levels in WT mice during caerulein-induced AP. (A) NAD⁺ and NADH were extracted from pancreatic tissues, and changes in NAD⁺ levels were measured using the NAD⁺/NADH assay kit. (B) PARP activity was assayed using the PARP assay kit. (C) PARP-1 activation was analysed by western blotting using anti-PAR. (D,E) Isolated acinar cells were treated with the PARP inhibitor, ABT-888 (1 μM), and caerulein (1 μM) for 24 h, and then the NAD⁺ level and SIRT1 activity were measured using the NAD⁺/NADH assay kit and SIRT1 assay kit. Each value represents the mean ± SD (n = 5). *P < 0.05.