Figure 4
From: NAD+ augmentation ameliorates acute pancreatitis through regulation of inflammasome signalling
Effect of β-Lap on pancreatic SIRT1 activity in WT mice during caerulein-induced AP. (A) Pancreatic tissue was isolated 11 h after the final injection of caerulein, and then SIRT1 activity was measured using a SIRT1 assay kit. (B,C) SIRT1 mRNA and protein levels in pancreatic tissue were measured by qRT-PCR (B) and western blotting (C). (D) miR-34a, miR-181, miR-9, and miR-146 were analysed by qRT-PCR. (E,F) Acetylated p53 and total p53 were detected by western blotting using anti-acetylated p53 and anti-p53 in WT (E) and SIRT1−/− mice (F). (G) Isolated acinar cells were treated with lentiviral particles containing control or p53 specific shRNA for 12 h. Cells were further treated with caerulein (1 μM) for 24 h, then the miR-34a level was measured by qRT-PCR. SIRT1 and p53 levels were determined by western blotting. Each value represents the mean ± SD (n ≥ 3). *P < 0.05, **P < 0.01.
