Figure 5 | Scientific Reports

Figure 5

From: Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening

Figure 5

Effect of compound 1 in an in vivo murine model of LPS/D-Galactosamine induced shock. (a) Mice survival after force-feeding with different doses of compound 1 and an intraperitoneal injection of LPS/D-Galactosamine. Groups of eight mice were used. Values are mean ± s.e.m. (b) TNFα levels in mice sera three hours after the induction of the shock by LPS/D-Galactosamine. Mice were forced-fed with 5 mg compound 1 in DMSO. Groups of eight mice were used. Values are mean ± s.e.m. (c) Aspartate aminotransferase (AST) levels in mice sera eight hours after the induction of the shock. Mice were forced-fed with 5 mg compound 1 in DMSO. Values are mean ± s.e.m. (d) Alanine aminotransferase (ALT) levels in mice sera eight hours after the induction of the shock. Mice were forced-fed with 5 mg compound 1 in DMSO. Values are mean ± s.e.m. (e) Livers sections (H&E staining) from mice forced-fed with DMSO (control) or compound 1 or injected with etanercept and an intraperitoneal injection of LPS/D-Galactosamine. (f) Liver sections from mice forced-fed with DMSO (control) or compound 1 or injected with etanercept and an intraperitoneal injection of LPS/D-Galactosamine. Sections were incubated with an anti-cleaved caspase-3 antibody and revealed with an anti-rabbit antibody and DAB.

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