Figure 1 | Scientific Reports

Figure 1

From: Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway

Figure 1

CRL-2868 Exosomes characterization (a) Detection by western blotting of ALIX, TSG 101 and CD63 in 30 µg of exosomes purified conditioned medium of CRL-2868 cells compared to 30 µg of parental cells whole lysate. Original uncropped WBs were reported in Figure S5A. (b) Representative negative EM image of exosomes released by CRL-2868 cells. Scale bar 500 nm. (c) Detection by western blotting of AREG in 30 µg of CRL-2868 exosomes compared to 30 µg of parental cells whole lysate. Original uncropped WBs were reported in Figure S5B. (d) Gold labelling immune-electron microscopy and negative staining indicating the presence of AREG antigen on exosomes. Scale bar 200 nm.

Back to article page