Figure 7
Neutralizing AREG antibodies revert osteoclast differentiation induced by NSCLC-exosomes. (a) TRAP staining of RAW 264.7 cells incubated with: CRL-2868 exosomes, Rec-AREG, CRL-2868 exosomes plus AREG neutralizing antibodies, for 6 days, stained for TRAP and compared with untreated cells (Ctrl). Scale bar 10 µm. (b) Evaluation by real Time PCR analysis of mRNA expression of TRAP and MMP9 in RAW 264.7 cells treated, for 6 days, with: CRL-2868 exosomes, Rec-AREG, AREG neutralizing antibodies, RANKL, CRL-2868 exosomes plus AREG neutralizing antibodies. (c) MMP9 protein levels assessed by ELISA, in RAW 264.7 cells treated, for 6 days, with: CRL-2868 exosomes, Rec-AREG, AREG neutralizing antibodies, RANKL, CRL-2868 exosomes plus AREG neutralizing antibodies. Values are the mean ± SD of three independent experiments *p ≤ 0.05, **p ≤ 0.01.
