Figure 5

Probe capture sequencing alignment is shown for individual capture pools (HPV16, O, B, F and P). HPV16 capture probe comprised of set of HPV16 specific probes, O capture probes consisted of certain viral and bacterial probes, B pool comprised of bacterial probes, F consisted of fungal probes and P comprised of parasitic probes that are mentioned in Table S3. The hybridization signals of the HPV probes used for the capture are shown as heat map in the figure. Six pools of whole genome and transcriptome amplified DNA plus cDNA was hybridized to a set of biotinylated conserved and specific viral probes, then captured on streptavidin beads, and used for tagmentation library preparation and deep sequencing with paired –end 250-nt reads. The miseq reads from individual capture when aligned with the metagenome of PathoChip (Chip probes) was found to cluster mostly at the capture probe regions. The genomic location along with the number of miSeq reads are mentioned in the figure for each organism.