Figure 4 | Scientific Reports

Figure 4

From: Beta sitosterol and Daucosterol (phytosterols identified in Grewia tiliaefolia) perturbs cell cycle and induces apoptotic cell death in A549 cells

Figure 4

BGT promotes caspase dependent apoptosis in A549 cells. (a) Scanning electron microscopic images of A549 cells treated with indicated concentration of BGT at 48 h. (b) western blot analysis of BGT treated A549 cells indicated concentration at 48 h incubation. β-actin was used as internal control and expression level of cleaved PARP, cleaved caspase-3, cleaved caspase-9, Bcl-2 and Bax was studied in BGT treated and untreated groups. (c) Expression level of intrinsic apoptotic proteins that was measured using ImageJ software analysis. (d) Expression level of pro and anti-apoptotic proteins.

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