Figure 5
From: Shear stress induces endothelial-to-mesenchymal transition via the transcription factor Snail
Snail was induced by low oscillatory shear stress in murine carotid arteries. Flow-altering, constrictive cuffs were placed on the right carotid arteries of C57BL/6 mice. They generated anatomically distinct regions exposed to high or low oscillatory wall shear stress (WSS; as indicated). Contralateral arteries were used as an experimental control. Experimental arteries were harvested after 14 days and en face staining was performed using anti-Snail antibodies (red), anti-CD31 antibodies (green) and the nuclear counter stain TO-PRO-3 (blue). Representative images (scale bar, 10 μm) and quantitation of Snail expression (n = 3; mean +/− SEM) are shown.
