Table 1 List of bacteria and plasmid used in this study.
Bacteria | ||
|---|---|---|
NTHI strain 723 | Otitis media clinical isolate | |
modA2 locked ON | NTHI strain 723 with 1 repeat in modA2, unable to phase vary | This Study |
modA2 locked OFF | NTHI strain 723 with 0 repeats in modA2, unable to phase vary | This Study |
modA2 locked ON:gfp | modA2 locked ON with pMDC-1 | This Study |
modA2 locked OFF:mCherry | modA2 locked OFF with pKM1.1 | This Study |
ΔoxyR modA2 locked ON | modA2 locked ON that lacks oxyR | This Study |
ΔoxyR modA2 locked OFF | modA2 locked OFF that lacks oxyR | This Study |
ΔkatA modA2 locked ON | modA2 locked ON that lacks katA | This Study |
ΔkatA modA2 locked OFF | modA2 locked OFF that lacks katA | This Study |
Plasmids | ||
pMDC-P1 | ompP5 promoter driving expression of gfpmut3 | |
pKM1.1 | ompP2 promoter driving expression of mCherry | |
pET46 Ek/LIC | LIC-compatible derivative of pET-46b(+) | EMD Millipore |
pET46::modA2-16reps | pET46 Ek/LIC containing the wild type modA2 (16 reps) of NTHI strain 723 | This Study |
