Figure 1 | Scientific Reports

Figure 1

From: High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway

Figure 1

High-throughput Screening Using a Fluorescence-labeled Substrate to Identify Effective MuPEP1 Inhibitors. (A) Sequence alignment of ComCs from S. pneumoniae, S. cristatus, and S. mutans and the structure of tCComC-AFC. Highly conserved hydrophobic amino acid residues and the consensus Gly–Gly are colored red. The arrowhead indicates the cleavage site of ComC by PEP. (B) Flow chart of high-throughput screening to identify compounds that inhibit the MuPEP1 activity and the biofilm formation of S. mutans. Results of the first screening are shown (upper right). The broken line indicates 50% inhibition. An example of the results from the third screening (in triplicate at two different compound concentrations) is also shown (lower left). The asterisks show that hit compounds were narrowed down by visual inspection of compound structures.

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