Figure 2

Cisplatin based barcoding uniquely codes eight samples with or without permeabilization eliminating sample-to-sample variability. (a) Eight PFA-fixed, methanol-permeabilized H9 cell samples were labeled with eight combinations of the Pt194, Pt195 and Pt196 cisplatin regents, run sequentially, pooled in silico and processed by density peak based deconvolution into individual samples as indicated. (b) Quantification of misassigned cells resulting from clustering of pooled barcoded data of known sample identity as number of cells per cluster is varied. (c) Eight PFA-fixed mouse bone marrow samples were labeled with eight combinations of the Pt194, Pt195 and Pt196 cisplatin regents, half of the samples were labeled with Rh103 intercalator, all samples were pooled, analyzed then separated by density peak based deconvolution into individual samples as indicated. (d) Rh103 signal intensity for each cluster identified in (c) shown with its corresponding barcode and expected Rh103 status. (e,f) Histograms, shown overlapping and individually, for OCT4 and SOX2 abundance compared across eight uniquely barcoded H9 cell samples split from the same sample and pooled prior to antibody staining.