Figure 3
From: Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
Cisplatin based barcoding used in conjunction with palladium barcoding enables a high degree of CyTOF sample multiplexing. (a) Two stage full binary and doublet filtering barcoding schemes utilizing cisplatin in combination with palladium enables a high degree of sample multiplexing. Two proposed barcoding schemes utilizing cisplatin based barcoding early in the sample processing pipeline prior to permeabilization to reduce the effective number of samples being handled and to permit multiplexing of 512 samples or doublet filtering multiplexing of 126 samples when used in conjunction with palladium barcoding. (b) Debarcoded 60-plex samples barcoded with a combination of platinum and palladium based barcoding. Each sample possesses a unique barcode that corresponds to the sample specific applied barcode.
