Figure 4 | Scientific Reports

Figure 4

From: SLC4A11 depletion impairs NRF2 mediated antioxidant signaling and increases reactive oxygen species in human corneal endothelial cells during oxidative stress

Figure 4

Depletion of SLC4A11 impairs NRF2 mediated antioxidant responses in immortalized human corneal endothelial cells. HCENCi were transfected with SLC4A11 siRNA (SLC-siRNA) or nonspecific control siRNA (C-siRNA) and exposed to 500 μM tBH for 4 h, and fold changes in gene expressions were determined by quantitative PCR. The depletion of SLC4A11 (a), and changes in expression of NRF2 (b), and antioxidant genes HO-1, and NQO1 (c) were examined. The activation of pIkB, pp38, pJNK and pERK was analyzed by immunocytochemistry (d). The experiments a, b, c was done three times in duplicates and experiment d was performed two times.

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