Figure 3 | Scientific Reports

Figure 3

From: Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-β/Smad Pathway and Osseointegration in Osteopenic Rabbits

Figure 3

(A) ALP activity and (B) Col-I and (C) OCN levels of BM-PCs cultured on Ti-6Al-4V (control) and on the CaSiO3, Zn-Ca 0.1 and Zn-Ca 0.3 coating surfaces with or without the osteogenic medium at 1, 7, 14 and 21 days. (D) Semi-quantitative analysis of in vitro matrix deposition mineralization was performed at 21 days by Alizarin red S staining. Negative control: BM-PCs on Ti-6Al-4V with growth medium. Positive control: BM-PCs on Ti-6Al-4V with osteogenic medium. Each value represents the mean ± SD from triplicate determinations.*p < 0.05, **p < 0.01, ***p < 0.001.

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