Figure 3
Production of RRPs from the WNV replicon plasmid by transfecting (a) BWNV-ME and (b) BWNV-CME cell lines. Green florescence was visualized when the replicon plasmid pWNVrepdCME-GFP was transfected into BWNV-ME cells (2), BHK-21 cells (11), BWNV-CME cells (10) or BWNV-ME cells together with the pCAG-WNV-C plasmid (1). The transfected cell supernatants were passaged onto fresh cell cultures as indicated by the arrows, and green florescence was observed only in the cells labelled 4 and 12. Three days post-inoculation, the supernatants of the cells infected first were used to inoculate the cells as indicated by the arrows for a second infection. Green florescence was analysed 72 h post-infection.
