Figure 3
C2238/αANP induces platelet aggregation and oxidative stress by the inhibition of cAMP levels and activation of Nox2. (A–D) Platelets were incubated without the peptide or with C2238/αANP at the specified concentration (10−6 M or 10−10 M). Forskolin (10 µM) or sNOX2-tat (50 µM) were also used where specified. After incubation and platelet stimulation with a sub-threshold concentration of collagen (0.3 µg/ml), platelet aggregation (A), soluble CD40 ligand release (B), platelet hydrogen peroxide levels (C) and platelet Nox2 activity were assessed. N = 12. The results were expressed as mean ± SEM. In all the groups, a similar volume of solvent was added.
