Figure 1
From: Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg
Expression of C5aR is increased and is associated with Treg reduction in cGVHD patients. (A) Representative flow cytometry plots showed C5aR expression in CD14+ monocytes from healthy controls, non-GVHD patients, or cGVHD patients. The statistical results revealed that C5aR expression was significantly up-regulated on the monocytes in cGVHD patients compared with healthy controls as well as non-GVHD patients who had undergone HSCT. (B) The lymphocytes were gated, and C5aR expression on these cells was also markedly increased in cGVHD groups. (C) Representative flow cytometry plots showed CD25 and Foxp3 expression in CD4+ cells from healthy controls, non-GVHD patients, or cGVHD patients. (D) Spearman’s correlation coefficient analysis revealed no significant correlation between C5aR expression on monocytes and the frequency of Treg, although the two parameters appeared to be negatively related. (E) There was a significant negative correlation between C5aR expression on lymphocytes and the frequency of Treg. *p < 0.05, **p < 0.005, ***p < 0.001.
