Figure 2

Subretinal DL-AAA injection caused increased expression of mGluR5. (A) Representative immunostaining for mGluR5 (red) and CRALBP (green) in a PBS-treated retina. Colocalization of the two (right column) is indicated by brightness of grey pixels. Scale bar = 50 µm. (B) Same as A but for a DL-AAA treated retina. (C) Representative western blotting for mGluR5 expression 7 days following DL-AAA treatment. β-actin as loading control. (D) Quantification of mGluR5 protein expression by western blotting, 7 days following DL-AAA treatment (n = 3 per group). (E) mGluR5 mRNA expression 7 days after subretinal injection of either DL-AAA (or PBS control). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.