Figure 7
From: Improved retinal function in RCS rats after suppressing the over-activation of mGluR5
A macromolecular complex is formed by mGluR5, AQP4 and Kir4.1. (A) Representative western blotting showing the expression of Kir4.1 and AQP4 by retinas following the administration of DL-AAA, CHPG or MPEP, vs. PBS controls. GADPH is a loading control. (B) Quantification of AQP4 protein expression under the above conditions (n = 3 per bar). (C) The same as (B), but for Kir4.1 expression (n = 3 per bar). (D) Representative immunoprecipitation assay for mGluR5, with subsequent immunoblotting for Kir4.1 and AQP4. Neg. Con., IgG negative control. Retinal lysate was used as a positive control. Bar charts show means ± SEM. **p < 0.01.
