Figure 8
From: Improved retinal function in RCS rats after suppressing the over-activation of mGluR5
Inhibition of mGluR5 by subretinal injection of MPEP restored the ERG b-wave amplitude and decreased the expression of GFAP in RCS rats. (A) Representative western blotting of mGluR5 expression at P20, P30, P60 and P90 in RCS (left) and RDY (right) rats. (B) Quantification of mGluR5 protein expression in RCS rats (red bars) vs. RDY rats (blue bars) at P20, P30, P60 and P90 (n = 3 for each bar). (C) Quantified mGluR5 mRNA expression in RCS vs. RDY rats at P20, P30, P60 and P90 (n = 3 for each bar). (D) Example ERG waveforms recorded from MPEP-injected eyes (red traces) vs. PBS (control)-injected eyes (blue) of RCS rats (P35) at 4 days post-injection. (E) Left: Average stimulus-response curves for ERG a-wave amplitude in the MPEP-group (red) vs. PBS-group (blue) at increasing light intensities (n = 7 for each data point). Right: The same for b-wave amplitude. (F) Amplitude of b-wave (light intensity: 0.5 log(cd*m/s2)) following MPEP (red) and PBS (blue) injection at 2, 4, 7, 14, 21 and 28 days post-injection. (G) Example immunolabelling with GFAP (red) and DAPI (gray) in the retinas of a PBS-injected eye (left) and an MPEP-injected eye (right) at 4 days post-injection. Scale bar = 50 µm (H) Quantified mRNA expression of rhodopsin, GS, Kir4.1, AQP4 and GFAP in MPEP-injected eyes (red) and PBS-injected eyes (blue) at 4 days post-injection (n = 3 per bar). Bar and line graph data show mean ± SEM. *p < 0.05, **p < 0.01. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.
