Table 1 Antibodies used in immunofluorescence staining, western blotting and immunoprecipitation.
From: Improved retinal function in RCS rats after suppressing the over-activation of mGluR5
Antibody | Company | Titre | Species | Cat# | Purpose |
|---|---|---|---|---|---|
Gαq | Santa Cruz | 1:50/1:100 | rabbit | sc-393 | IF/WB |
PLCβIII | Santa Cruz | 1:50/1:100 | rabbit | sc-403 | IF/WB |
mGluR1 | Millipore | 1:500/1:1000 | rabbit | mab9448 | IF/WB |
mGluR5 | Abcam | 1:500/1:1000 | rabbit | ab53090 | IF/WB |
mGluR5 | Abcam | 1:100/1:200 | rabbit | ab27190 | IP |
AQP4 | Abcam | 1:100 | mouse | ab9512 | WB |
Kir4.1 | Santa Cruz | 1:100 | goat | sc-23637 | WB |
GFAP | Abcam | 1:500 | rabbit | Ab7260 | IF |
GS | Millipore | 1:600 | mouse | mab302 | IF/WB |
CRALBP | Abcam | 1:200 | mouse | ab15051 | IF |
GAPDH | Cwbio, China | 1:2000 | rabbit | cw0101 | Internal control |
β-actin | Cwbio, China | 1:1000 | mouse | cw0096 | Internal control |
Secondary antibodies Cy3 (mouse) | Beyotime, China | 1:500 | goat | A0521 | IF |
Secondary antibodies Cy3 (rabbit) | Beyotime, China | 1:500 | goat | A0516 | IF |
Secondary antibodies Cy3 (goat) | Beyotime, China | 1:500 | donkey | A0502 | IF |
Secondary antibodies Cy3 (mouse) | GeneTex | 1:500 | donkey | GTX85338 | IF |
Secondary antibodies FITC (goat) | Santa Cruz | 1:200 | donkey | SC-2024 | IF |
Secondary antibodies FITC (mouse) | Zhongshan Goldenridge, China | 1:200 | goat | ZF-0312 | IF |
