Figure 3
From: Structural Characterization of the SMRT Corepressor Interacting with Histone Deacetylase 7
Determination of SMRT(1255–1452)–HDAC7 binding affinity. (a) Surface plasmon resonance curves of 8, 6, 2, 1, and 0.5 µM HDAC7 (upper to lower curve) binding to immobilized SMRT(1255–1452), yielding a Kd of 2.1 µM. Dashed curves represent fits to the data (solid lines) using a three-state (two-step) binding model. (b) Microscale thermophoresis data of HDAC7 binding to fluorescently labeled SMRT. The solid line represents a fit to the data yielding a Kd of 2.8 µM. Vertical axis shows the ratio of fluorescence (F) measured with and without laser heating.
