Figure 4
From: Structural Characterization of the SMRT Corepressor Interacting with Histone Deacetylase 7
NMR analysis of SMRT(1255–1452). (a) Secondary structure propensity (SSP) values based on experimental Cα and Cβ chemical shifts. Positive values indicate α-helical, negative values β-extended propensity. Cutoff values of 0.1 are indicated by horizontal dashed lines. (b) 1H-15N heteronuclear nuclear Overhauser enhancement (hetNOE) values at 25 °C and 600 MHz 1H Larmor frequency. (c) Transverse (R 2) 15N relaxation rates measured at 25 °C and 600 MHz 1H Larmor frequency. Regions with SSP values above 0.1 and elevated hetNOE values, indicative of transient helical structure, are indicated with gray shading. Error bars in panels (b) and (c) represent parameter standard deviations estimated based on noise levels in NMR spectra. Residues without data in panels (b) and (c) are prolines (which do not appear in HSQC-type spectra), have overlapping resonance signals, or are unassigned due to insufficient signal intensity.
