Figure 8
From: Structural Characterization of the SMRT Corepressor Interacting with Histone Deacetylase 7
Test of SMRT(1255–1452) binding to the C535A/H541A mutant of HDAC7. (a) Intensities of resonance signals of SMRT(1255–1452) residues in 15N-1H HSQC NMR spectra with added 3.6-fold molar excess of the HDAC7 (C535A/H541A) mutant, normalized to corresponding intensities in the HSQC spectrum of free SMRT(1255–1452). Compare to Figure 5 for WT HDAC7. Missing bars correspond to proline residues, residues with overlapping resonance signals, or unassigned residues. (b) Microscale thermophoresis measurement of the interaction of SMRT(1255–1452) with the HDAC7 (C535A/H541A) mutant. See Fig. 3b for WT HDAC7. (c) Western blot of pull-down assay testing HDAC7 (C535A/H541A) binding to the three GST fusion peptides derived from SMRT(1255–1452). Results of the same assay for WT HDAC7 are reproduced from Fig. 6a for comparison.
