Figure 2
From: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
The c-Myc-TCRP1 axis inhibites sensitivity of tongue and lung cancer cells to cDDP. (A) Transfected plasmid pLEX-TCRP1 and its control into Tca8113 cells, meanwhile transfected siRNAs targeting TCRP1 and its control into Tca8113/PYM cells, 48 h later, western blot assay was used to measure TCRP1 expression levels in these different treated cells. (B) Overexpressed c-Myc together with silencing TCRP1 in parative cells Tca8113 and A549, (C) Silencing c-Myc together with over-expressed TCRP1 in chemoresistance cells Tca8113/PYM and A549/DDP, all these cells and each control group were treated with cDDP for 72 h, and the survival rate of these cells were detected by MTS assay, western blot assay was used to measure Akt activation. **P < 0.01.
