Figure 1
From: Thymosin beta-4 regulates activation of hepatic stellate cells via hedgehog signaling
Knockdown of TB4 inhibits the proliferation, migration and activation of HSCs. (a) Cell proliferation were measured by MTS assays in LX-2 cells with or without scramble (Con) or TB4 siRNA. The mean ± s.e.m. results obtained from three independent experiments are graphed (b,c) Cell migration were measured by wound healing assays in Con siRNA- or TB4 siRNA-transfected LX-2 cells. Artificial wounds were created on cells in confluence. Images were taken at 0, 24, 36 and 48 hours after wound. Data shown represent one of three experiments with similar results and the mean ± s.e.m. results obtained from three repetitive experiments are graphed (b: Representative images, c: Quantification of wound closure) (d,e) Protein expression of ASMA in these cells. Data shown represent one of three experiments with similar results and the mean ± s.e.m. results obtained from three repetitive experiments are graphed (GAPDH was used as an internal control) (d: Immunoblot, (e: Band density) (*p < 0.05; **p < 0.005).
