Figure 2 | Scientific Reports

Figure 2

From: Bone morphogenetic protein 4 promotes the survival and preserves the structure of flow-sorted Bhlhb5+ cochlear spiral ganglion neurons in vitro

Figure 2

Isolation of Bhllhb5-tdTomato+ SGNs via flow cytometry: (A) The modiolus harboring SGNs was dissected from Bhlhb5-tdTomato+ mice and trypsinized to dissociate it into single cells. The tdTomato+ and tdTomato− cells were sorted via flow cytometry. (B) Immunostaining of Bhlhb5-tdTomato+ cells after sorting showed a high percentage of Tuj1+ (93.3%) and tdTomato+ (96.4%) cells but no Sox2+ (0.0%) cells. (C) Immunostaining of Bhlhb5-tdTomato− cells displayed a high percentage of Sox2+ (97.1%) cells but no Tuj1+ or tdTomato+ cells. (D) Quantitative PCR results showed the relatively higher expression of Tuj1 in Bhlhb5-tdTomato+ cells, while the Bhlhb5-tdTomato− cells had relatively higher expression of Sox2. **p < 0.01. Scale bars are 20 μm in (B and C).

Back to article page