Figure 3 | Scientific Reports

Figure 3

From: Bone morphogenetic protein 4 promotes the survival and preserves the structure of flow-sorted Bhlhb5+ cochlear spiral ganglion neurons in vitro

Figure 3

Exogenous BMP4 treatment promoted the survival of flow-sorted Bhlhb5-tdTomato+ SGNs: (A) The protocol for how Bhlhb5-tdTomato+ cells from the modiolus were sorted by flow cytometry and cultured for 7 days with different concentration of BMP4 or Noggin. (B–F) Immunofluorescence imaging of Bhlhb5-tdTomato+ SGNs after treatment with the different concentration of BMP4 or Noggin. There were greater numbers of monopolar neurons (arrowheads) in the BMP4-treated groups compared to the controls, and this number decreased significantly after treatment with Noggin. (G) Quantification of the number of surviving SGNs after 7 days of culture. (H) Measurement of the neurite lengths extending from the monopolar and bipolar SGNs after 7 days of culture. *p < 0.05, **p < 0.01. Scale bars are 20 μm in (B–F).

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