Figure 2
From: Nanotopological plate stimulates osteogenic differentiation through TAZ activation
The O70 nanopattern plate is optimal for TAZ nuclear localization and activation. (A) Features of the O60, O70, and O80 nanopattern plates. Feature diameter, gap length between features, and the distance of the center-to-center were listed, and the SEM image of each nanopattern plate is shown. (B) MSC were seeded on flat or nanopattern plates and harvested after 48 hr. Transcript levels of CTGF and CYR61 were analyzed by quantitative real-time PCR. Asterisks mean statistical significance. n = 3, **p < 0.01, ***p < 0.005, ns = not significant, t-test. (C) MSC were transfected with CTGF-luc. After 16 hr, cells were re-seeded on flat or nanopattern plates, and luciferase activity was measured using a luciferase reporter gene assay system 24 hr after re-seeding. n = 3, ***p < 0.005, t-test. (D) MSC were seeded on flat or nanopattern plates and immunostained with TAZ specific antibody 24 hr after seeding. F-actin and the cell nucleus were counterstained with phalloidin and DAPI, respectively. (E) Immunostained cells in panel (D) were counted using the image J software, and the cell population for nuclear dominant, cytosol dominant, and evenly distributed TAZ was calculated. n = 3, *p < 0.05, ***p < 0.005, t-test.
