Figure 5
From: Nanotopological plate stimulates osteogenic differentiation through TAZ activation
Inhibition of Rho/ROCK/F-actin represses TAZ nuclear localization and TAZ target-gene expression induced by nanopattern plates. (A) MSC were seeded on O70 nanopattern plates and 24 hr after seeding, latrunculin A, Y27632, and Rac1 inhibitors were added for 4 hr. Cells were stained with TAZ specific antibody and counterstained with phalloidin and DAPI for visualization of filamentous actin and nucleus, respectively. (B) Cells in panel (A) were harvested and total RNA was prepared. TAZ target gene CTGF was analyzed by qRT-PCR. n = 3, ***p < 0.005, t-test.
