Figure 1

Effect of dietary folate restriction before pregnancy on body weight gain, food consumption and biochemical parameters in mice. (a) Study design illustrating the period of folate deficiency in mice. (b) ^#Preconceptional body weight gain and (c) food intake were largely unaffected. (d) Fasting blood glucose was not influenced by folate deficiency but (e) serum folate levels were decreased by feeding a folate deficient diet for 6 weeks. Values are given as mean + SEM; *P < 0.05 vs. control; unpaired Student’s t-test; n = 6–10/each group; ^#Two-way repeated-measure ANOVA and post-hoc pairwise comparisons with Bonferroni correction (diet × weight) did not reveal any significant interaction between folate diet and weight gain at different time points (F = 0.958, p = 0.45, n = 10 each group).