Figure 6

Effect of maternal folate deficiency on maternal tissue growth and mTOR signaling. (a) Maternal liver and (b) heart, spleen, kidney weights were decreased in folate deficient dams. Values are given as mean + SEM; *P < 0.05 vs. control; unpaired Student’s t-test; n = 6–7. (c) mTORC1 (S6-S-235/236, S6, 4E-BP1-T-37/46) and mTORC2 (Rictor, Akt-S-473) downstream signaling in maternal skeletal muscle was inhibited in folate deficient dams. Using Western blot analysis, two distinct bands were observed for total 4E-BP1 in maternal muscle and analyzed both bands together. Histogram (d,e) summarizes the immunoblot data of maternal skeletal muscle mTORC1and mTORC2 signaling. Values are given as mean + SEM; *P < 0.05 vs. control; unpaired Student’s t-test; n = 6–7. Full-length gels and blots are included in the Supplementary Information File.