Figure 1 | Scientific Reports

Figure 1

From: LEA29Y expression in transgenic neonatal porcine islet-like cluster promotes long-lasting xenograft survival in humanized mice without immunosuppressive therapy

Figure 1

Reconstitution of human immune system and production of antibodies in NSG mice (HU-SRC-SCID) transplanted with wild-type (Tx-wt) or INSLEA29Y transgenic (Tx-LEA-tg) NPICCs. Isolated human CD34+ hematopoietic stem cells were transferred to NSG mice. After 16–20 weeks and at the end of the observation period the percentage of human immune cells (hCD45+) and immune cell subsets (gated on human CD45) were analyzed by FACS (A). Xenoantibodies (B) and plasma levels of human IgG and human IgM (C) were measured by ELISA.

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