Figure 3 | Scientific Reports

Figure 3

From: LEA29Y expression in transgenic neonatal porcine islet-like cluster promotes long-lasting xenograft survival in humanized mice without immunosuppressive therapy

Figure 3

Histological analysis of transplanted NPICCs. Islet xenografts were investigated at day 30 and day 120 post Tx. (A) Staining for insulin (red) revealed that wild-type NPICCs were rejected and displayed a strong infiltration with human CD45+ (brown) immune cells. LEA29Y expressing islets were protected from rejection and exhibited a preserved islet xenograft with few infiltrating CD45+ cells. (B) Transplanted wild-type NPICCs at day 20–30 post Tx stained against insulin (red) and human CD45, CD3, CD4, CD8, CD68, and FoxP3 (brown): only few insulin+ cells (arrows) are detectable but massive immune cell accumulation whereby infiltrating cells mostly consist of hCD4+ and hCD8+ lymphocytes, some macrophages, and few FoxP3+ T cells. Scale bar: 100 μm.

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