Figure 5
From: Improved Growth Patterns in Cystic Fibrosis Mice after Loss of Histone Deacetylase 6
Increased KLF2 expression in CF cultured pre-adipocytes and primary inguinal fat. (a) Pre-adipocyte 3T3-L1 cells with CFTR expression ablated (CF) as described in Methods exhibit significantly decreased acetylated α-tubulin (Ac-tub) and increased KLF2 compared to control (WT) cells from each individual mouse tested. Significance between groups was determined by t-test (*p < 0.001; n = 6 for Ac-tub; p = 0.001; n = 9 for KLF2); (b) Representative gel showing response of Ac-tub and KLF2 expression in WT and CF 3T3-L1 cells to doses of HDAC6 inhibitor tubastatin. Representative of duplicate experiments. (c) KLF2 expression in inguinal fat from WT, CF, HDA, and CF/HDA mice. Representative gels showing ac-tub and KLF2 expression is shown. At right, a scatter plot shows values from each individual mouse tested (*p < 0.05 compared to CF v. CF/HDA by ANOVA with Newman-Keuls multiple comparison test). Gels are cropped for space. Full-length gels are provided in Supplemental Data.
