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Figure 2

From: Mutations in human AID differentially affect its ability to deaminate cytidine and 5-methylcytidine in ssDNA substrates in vitro

Figure 2

Analyses of enzyme’s concentration-dependent deamination of C (a) and 5 mC (b) containing substrates by wt hAID and N51A mutant. The graph presents the dependence between the yield of C/5 mC deamination reactions and enzyme concentration (wt hAID (triangles) and N51A mutant (circles)). UDG-coupled and TDG-coupled deamination assays (see Methods) were used for C and 5 mC substrates, respectively. Error bars represent standard deviation from the mean of at least three independent replicates. Representative PAGE analyses of the products of deamination reaction are shown on the right. Positive and negative control reactions are indicated by “+” or “−”, respectively. The cropped gel images are displayed. Full-length gel images are shown in Supplementary Fig. S7.

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