Figure 3 | Scientific Reports

Figure 3

From: Mutations in human AID differentially affect its ability to deaminate cytidine and 5-methylcytidine in ssDNA substrates in vitro

Figure 3

Representative results obtained during the analysis of wt hAID (a) or N51A mutant (b) deaminase activities on all possible combinations of NNCN sequences (20 clone sequences for NNCN_1 oligonucleotide; results for NNCN_2, 3, and 4 oligonucleotides are shown in Supplementary Fig. S3). The first line of each alignment contains the sequence of the NNCN_1 oligonucleotide untreated with hAID. Substitutions are indicated by one-letter symbols corresponding to a particular nucleotide. Positions identical to those in the untreated sequence are marked by dots. Deletions/insertions presumably caused by the used polymerase are marked by “~”. Flanking sequences – 20-nt-long PCR primer binding sites are not shown in the figure.

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