Figure 2
From: Mahanine exerts in vitro and in vivo antileishmanial activity by modulation of redox homeostasis
Mahanine-mediated mitochondrial dysfunction, ROS generation, and ROS-dependent cell death in AG83 virulent promastigotes. (a) Promastigotes (2 × 106) were preloaded with H2DCFDA (50 µM) and plated in a six-well plate. The IC90 dose of mahanine (25 µM) was used at different time points (0–1 hr). H2DCFDA positivity was measured by FACS. (b) Promastigotes were preloaded with H2DCFDA and treated with mahanine (0–30 µM) for 45 min. For the highest concentration of mahanine (30 µM) treatment, promastigotes were pretreated with NAC (20 mM). Cells were acquired in FACS and analyzed. (c) Promastigotes (2 × 106) were either untreated or treated with NAC (20 mM) and incubated with mahanine (0–15 µM) for 24 hr at 22 °C. Promastigotes were stained with PI and analyzed in FACS. (d) A representative image was shown by a histogram. (e) Promastigotes were either treated with ethanol (1%) or mahanine (0–30 µM) for 24 hr at 22 °C.Parasites were incubated with JC1 for 30 min, processed and analyzed by FACS.
