Table 3 Kinetic parameters of the Tm1743 double mutants.

From: Semi-rational engineering of a thermostable aldo–keto reductase from Thermotoga maritima for synthesis of enantiopure ethyl-2-hydroxy-4-phenylbutyrate (EHPB)

Enzymes

K m (mM)

k cat (s−1)

k cat /K m (s −1mM−1)

Wild-type

1.10 ± 0.04

1.96 ± 0.02

1.78 ± 0.03

W21L/W118E

10.53 ± 0.10

1.98 ± 0.02

0.19 ± 0.01

W21L/W118H

15.98 ± 0.20

1.98 ± 0.01

0.12 ± 0.01

W21S/W86E

1.25 ± 0.02

1.01 ± 0.01

0.81 ± 0.02

W21S/W86N

1.62 ± 0.02

0.84 ± 0.01

0.51 ± 0.02

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