Figure 1
Establishment of resistant and susceptible monoclonal cells. (A) Single cell sorting by flow cytometry. The black spots mark dead cells in sample, the red and green spots mean adhesive cells, above cells were abandoned to ensure the vitality and purity of the sorting cells. And the remaining blue spots signify the cells used for single cell sorting. (B) Cell proliferation assay. The straight line signifies CV of the control CIK cells, the monoclonal cells of which CV above the line were the candidate resistant cells, while those CV under the line were preliminarily identified as susceptible cells. (C) Antiviral activity assay. The antiviral activities of control (C1), resistant (R2) and susceptible (S3) cells were detected at 0, 6, 12, 24, 48, 72, 96 and 120 h post GCRV infection. (D) CPE analysis. The morphology and CPE of C1, R2 and S3 cell groups were recorded at 0, 6, 12, 24, 48, 72, 96 and 120 h post GCRV challenge.
