Figure 6
From: Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex
Otx2 infusion restores the plasticity and expression of CSPG. (A) VEP amplitudes of the first negative peak (mean ± SEM) and averaged traces (inset, for low or middle spatial frequency) reveal the acuity of the deprived eye in Otx2-infused T1 KO (black) or vehicle-infused KO mice (red). (B) Visual acuity of individual T1 KO mice (bottom) infused from P21 to P28 and deprived at P24 (top). Otx2-infused mice showed a significant acuity reduction in the deprived eye (black symbols, 0.27 ± 0.02 cycles/degree) compared to vehicle-infused KO (red symbols, 0.51 ± 0.02 cycles/degree, p < 0.0001, t-test). (C–F) Infused Otx2 (red) facilitates WFA staining (green) in T1 KO mice (D,F) compared to the opposite non-infused hemisphere in the same brains (C,E). Otx2-HA is internalized in WFA-labeled cells (inset in F compared to in E). The scale bars represent 100 μm. (G) Numbers of WFA-labeled cells are increased in Otx2-infused T1 KO mice (600 × 350 μm; numbers of cells, non-infused side 21.7 ± 1.05 versus Otx2-infused side 33.8 ± 2.3, six mice, p = 0.003, paired t-test). (H) Normalized mRNA expression of aggrecan in juvenile V1. Note that despite a specific decrease in aggrecan protein, the transcripts detected with RT-qPCR were not reduced in T1 KO (six mice, p > 0.05, t-test). Error bars represent SEM. (I) Co-immunoprecipitation (IP) of eIF4E and Otx2. Lanes from the left, input (1/40), supernatant (sup), IP with anti-eIF4E, anti-Otx2 antibody, normal IgG. The full-length blot in a Supplementary Information. (J) Association between Otx2 and aggrecan mRNA in juvenile V1. Three independent Otx2 immunoprecipitates and their initial input samples were analyzed for aggrecan, rpl271, and GAPDH mRNAs. Relative fold enrichment of each mRNAs is shown and compared to the input (hatched blue line).
