Figure 2

Time-resolved fluorescence of of Peredox in the NADH concentration titration experiment. Lifetime τ _i (a) and fractional amplitude α _i (b) of Peredox in the NADH concentration titration experiment. The α _i and τ _i were obtained from the fluorescence decay curves demonstrated in Supplementary Figure S2a. (c) Fractional intensities (α _i τ _i ) versus NADH concentrations. (d) Ratio of fractional intensities (\({R}={{\alpha }}_{1}{{\tau }}_{1}/({{\alpha }}_{2}{{\tau }}_{2}+{\alpha }{}_{3}{\tau }_{3})\)) and average lifetime versus NADH concentrations. The ratio of fractional intensities and average lifetime curves were fitted with Hill equation, and the parameters are presented in Supplementary Table S1. Error bars represent the standard deviation of the mean.