Figure 3
Inhibitory effect of serum derived from A. hydrophila DNA stimulated zebrafish on the growth of A. hydrophila. (A) Growth curve of A. hydrophila (A.h) in M9 medium cultured in the presence of unstimulated serum (Unstimulated), A. hydrophila DNA (1 μg per fish) stimulated serum (Stimulated), heat-treated stimulated serum (Heated), stimulated serum neutralized with anti-hepcidin Ab (Hepcidin Ab), stimulated serum neutralized with anti-hepcidin Ab plus 10 μM Fe2+ supplemented ones (Hepcidin Ab + Fe2+), and A. hydrophila cultured alone in M9 (Mock). (B) Detection of the bacterial generation time of A. hydrophila cultured in the presence of different serum samples (unstimulated, stimulated, and Hepcidin Ab + Fe2+) in M9 medium for 8 h. (C) Detection of the absorbance (A600) values of A. hydrophila cultured in the presence of different serum samples (unstimulated, stimulated, and Hepcidin Ab + Fe2+) in M9 medium at 8 h time point. All data are representative of at least three independent experiments. *p < 0.05, **p < 0.01.
