Figure 1
From: Proximal GATA-binding sites are essential for human HSD3B1 gene transcription in the placenta
Transcriptional activity of the human HSD3B1 promoter. (A) The human HSD3B1 (B1: −2226/+337) and HSD3B2 (B2: −1859/+556) promoter regions (shown on the top) were fused to luciferase reporter to generate promoter-luciferase constructs. “+1” is the transcription start site indicated by the arrow. The HSD3B1, HSD3B2 or empty (v) promoter-luciferase construct was transiently transfected into JEG-3 cells. (B) A series of 5′-deleted HSD3B1 promoter-luciferase constructs were transiently transfected into JEG-3 cells. Cells were harvested after 24 h of transfection and assayed for luciferase activity. Results are presented as the activity relative to the empty vector. Data are means ± SD of five (A) or three (B) independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with vector control. # P < 0.05.
